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Prevalence of Brucella Melitensis and Brucella Abortus in Raw Milk and Dairy Product by Real Time PCR Technique

Yaran Majid, Najafi Somayeh, Shoaei Parisa, Ataei Behrooz, Fadaei Nobari Reza, Ramazanpour Javad, Farsi Mehdi, Ahmadi Ahwaz Nasrin, Khorasani Marzieh.

Abstract
Introduction: Brucellosis is a public health problem with a high incidence. The main causes of acquiring brucellosis are through the consumption of unpasteurized milk and dairy products. In this study we described a real-time PCR assay for more sensitive, rapid detection and differentiation of Brucella.abortus and Brucella.melitensis in raw milk and dairy products in Isfahan province.
Materials and Methods: A Taq Man analysis and single step PCR (BruAb2_0168BMEII0466 genes) has performed in total of 132 unpasteurized milk samples and 65 dairy products. Samples were selected from various markets and animal farms located in different cities of Isfahan province. The duration period of sample collection was nearly two months (May to June 2012).
Results: By real-time PCR 4 of 132 milk specimens (3%) were positive for B. melitensis. Also one oil sample between 65 different dairy products (1.5%) was positive for B. melitensis. None of the specimens were positive for B. abortus. We detected direct and incomplete correlation between brucella infected milk and different cities(r=0.024, p value >0.05).
Conclusion: Results of present study indicated a relative high presence of this pathogen in raw milk. Real-time PCR is technically more simple, accurate, and rapid than current standard methods for identification of Brucella species .One of the most effective routes to control the disease includes pasteurization or boiling of milk and other dairy products for human consumption.

Key words: Brucellosis, real time PCR, milk


 
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How to Cite this Article
Pubmed Style

Majid Y, Somayeh N, Parisa S, Behrooz A, Reza FN, Javad R, Mehdi F, Nasrin AA, Marzieh K. Prevalence of Brucella Melitensis and Brucella Abortus in Raw Milk and Dairy Product by Real Time PCR Technique. Ulutas Med J. 2016; 2(1): 7-11. doi:10.5455/umj.20151004054235



Web Style

Majid Y, Somayeh N, Parisa S, Behrooz A, Reza FN, Javad R, Mehdi F, Nasrin AA, Marzieh K. Prevalence of Brucella Melitensis and Brucella Abortus in Raw Milk and Dairy Product by Real Time PCR Technique. www.scopemed.org/?mno=201961 [Access: November 24, 2017]. doi:10.5455/umj.20151004054235



AMA (American Medical Association) Style

Majid Y, Somayeh N, Parisa S, Behrooz A, Reza FN, Javad R, Mehdi F, Nasrin AA, Marzieh K. Prevalence of Brucella Melitensis and Brucella Abortus in Raw Milk and Dairy Product by Real Time PCR Technique. Ulutas Med J. 2016; 2(1): 7-11. doi:10.5455/umj.20151004054235



Vancouver/ICMJE Style

Majid Y, Somayeh N, Parisa S, Behrooz A, Reza FN, Javad R, Mehdi F, Nasrin AA, Marzieh K. Prevalence of Brucella Melitensis and Brucella Abortus in Raw Milk and Dairy Product by Real Time PCR Technique. Ulutas Med J. (2016), [cited November 24, 2017]; 2(1): 7-11. doi:10.5455/umj.20151004054235



Harvard Style

Majid, Y., Somayeh, N., Parisa, S., Behrooz, A., Reza, F. N., Javad, R., Mehdi, F., Nasrin, A. A. & Marzieh, K. (2016) Prevalence of Brucella Melitensis and Brucella Abortus in Raw Milk and Dairy Product by Real Time PCR Technique. Ulutas Med J, 2 (1), 7-11. doi:10.5455/umj.20151004054235



Turabian Style

Majid, Yaran, Najafi Somayeh, Shoaei Parisa, Ataei Behrooz, Fadaei Nobari Reza, Ramazanpour Javad, Farsi Mehdi, Ahmadi Ahwaz Nasrin, and Khorasani Marzieh. 2016. Prevalence of Brucella Melitensis and Brucella Abortus in Raw Milk and Dairy Product by Real Time PCR Technique. THE ULUTAS MEDICAL JOURNAL, 2 (1), 7-11. doi:10.5455/umj.20151004054235



Chicago Style

Majid, Yaran, Najafi Somayeh, Shoaei Parisa, Ataei Behrooz, Fadaei Nobari Reza, Ramazanpour Javad, Farsi Mehdi, Ahmadi Ahwaz Nasrin, and Khorasani Marzieh. "Prevalence of Brucella Melitensis and Brucella Abortus in Raw Milk and Dairy Product by Real Time PCR Technique." THE ULUTAS MEDICAL JOURNAL 2 (2016), 7-11. doi:10.5455/umj.20151004054235



MLA (The Modern Language Association) Style

Majid, Yaran, Najafi Somayeh, Shoaei Parisa, Ataei Behrooz, Fadaei Nobari Reza, Ramazanpour Javad, Farsi Mehdi, Ahmadi Ahwaz Nasrin, and Khorasani Marzieh. "Prevalence of Brucella Melitensis and Brucella Abortus in Raw Milk and Dairy Product by Real Time PCR Technique." THE ULUTAS MEDICAL JOURNAL 2.1 (2016), 7-11. Print. doi:10.5455/umj.20151004054235



APA (American Psychological Association) Style

Majid, Y., Somayeh, N., Parisa, S., Behrooz, A., Reza, F. N., Javad, R., Mehdi, F., Nasrin, A. A. & Marzieh, K. (2016) Prevalence of Brucella Melitensis and Brucella Abortus in Raw Milk and Dairy Product by Real Time PCR Technique. THE ULUTAS MEDICAL JOURNAL, 2 (1), 7-11. doi:10.5455/umj.20151004054235








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