Open Access and Peer-reviewed Medical Journal
Home Journal Issues Guide for Authors Editorial Board Aims & Scope About Journal


Original Research Open Access


A comparative study of diagnosis methods for detection of Helicobacter pylori in gastro-duodenal diseases in Human

Fiaz Ahamed, N.R. Chandrappa, Emad M. Abdallah.

Cited by (1)

Abstract
Background: Helicopacter pylori play an important role in the pathogenesis of gastroduodenal disease. Appropriate diagnosis of H.pylori infection in the laboratory results in relief of the symptoms and many also be cured without surgical intervention.
Method: 76 males and 24 females who underwent diagnostic endoscopy formed the study group. Microscopic examination, culturing, rapid urease test and ELISA test were carried out.
Results: Among 100 cases (study group) suffering from gastroduodenal diseases, H. pylori isolated from 18 cases, identified in 54 grams stained smears, rapid urease test was positive in 62 cases, IgG immunoglobulins against H. pylori was detected in 62 cases.
Conclusion: No single test can be considered sensitive or specific to detect or rule out H. pylori infection and it is necessary to use a combination of tests.

Key words: Keywords: Helicopacter pylori, diagnosis, gram staining, rapid urease test, ELISA.


 
ARTICLE TOOLS
Abstract
PDF Fulltext
Print this article Print this Article
How to cite this articleHow to cite this article
Export to
Export to
Related Records
 Articles by Fiaz Ahamed
Articles by N.R. Chandrappa
Articles by Emad M. Abdallah
on Google
on Google Scholar
Article Statistics
 Viewed: 734
Downloaded: 292
Cited: 1


REFERENCES
1. Marshall BJ, Warren JR. Unidentified curved bacilli in the stomach of patients with gastritis and peptic ulceration. Lancet. 1984, 1(8390):1311-1315. [DOI via Crossref]   
2. Steer H.W. Ultrastructures of cell migration through the gastric epithelium and its relationship to bacteria. J Clin Path. 1975, 28: 63-70. [DOI via Crossref]   
3. Subbukesavaraja V.,.Balan K. Comparative study of invasive methods for diagnosis of Helicobacter pylori in humans. International journal of current microbiology and applied sciences. 2013, 2(7): 63-68.
4. International Agency for Research on Cancer: Shistosomes, Liver flukes and Helicobacter pylori.IARC Monographs on the evaluation of carcinogenic risk to humans. 1994. Vol.6 Iyon IARC.
5. Goodwin C.S., Armstrong J.A., Chivers T. et al. Transfer of Campylobacter pylori and Campylobacter mustelase to Helicobacter gen nov and H. pylori comb, nov and Helicobaacter mustelase comb. Nov, respectively. Int. J. Syst. Bacteriol. 1989, 39: 397-405. [DOI via Crossref]   
6. Hazell S.L., Barody T.J. Gal A., et al. C. pylori detection of urease as a marker of bacterial colonization and gastritis. Am. J. Gastroenterol, 1987. 82: 292-296. [Pubmed]   
7. Goodwin C.S., Armstrong J.A. Microbiological aspects of H. pylori. Eur. J. Clin. Microbiol. Infect. Dis., 1990, 9: 1-13. [DOI via Crossref]    [Pubmed]   
8. Skirrow M.B. Isolation of C. pylori antibiotic supplementation. British Medical Journal. 1990, 2-9.
9. Axon A.T.R. The transmission of Helicobacteri pylori, which theory fits the facts?.Eur. J. Gastroenterol. Hepatol. 1996. 8: 1-2. [DOI via Crossref]    [Pubmed]   
10. Goodman K.J., Correu P. The transmission of Helicobacter pylori. A critical review of the evidence. Int. J. Epidemiol. 1995, 24: 875-887. [DOI via Crossref]    [Pubmed]   
11. Suvak B., Dulger A.C., Suvak O., Aytemiz E., Kemik O. The prevalence of helicobacter pylori among dyspeptic patients in an earthquake-stricken area. Clinics. 2015, 70(1):69-72. [DOI via Crossref]   
12. Uotani T, Graham DY. Diagnosis of Helicobacter pylori using the rapid urease test. Annals of Translational Medicine. 2015, 3(1):9. [Pubmed]    [PMC Free Fulltext]   
13. Finegold S.M., Baron E.J. Bailey and scott's Diagnostic microbiology 7thed. C.V. Mosby Company, St. Louis, Toronto, Princeton.
14. Correa P, Houghton J. Carcinogenesis of Helicobacter pylori. Gastroenterology. 2007, 133(2):659-72. [DOI via Crossref]    [Pubmed]   
15. Mainmooma M., Habibullah C.M., Singh N. Evaluation of methods to detect H. pylori from human antral mucosa. 1994. Indian Journal of Medical Microbiology. 12(1): 39-41.
16. Schnell A.A., Schubert T.T. Usefulness of culture, histology and urease testing in the detection of C. pylori. Am. J. Gastroenterol. 1989, 84: 133-137. [Pubmed]   
17. Hazell, S. L., Lee, A., Brady, L., and Hennessy, W. Campylobacter pyloridis and gastritis: Association with intercellural spaces and adaptation to an environment of mucus as important factors in colonization of gastric epithelium. J Infect Dis. 1986, 153: 658-663. [DOI via Crossref]    [Pubmed]   
18. McNulty C.A., Dent J.C., Uff J.S., Gear M.W., Wilkinson S.P. Detection of Campylobacter pylori by the biopsy urease test: an assessment in 1445 patients. 1989. Gut, 30: 1058-1062. [DOI via Crossref]    [Pubmed]    [PMC Free Fulltext]   
19. Brown L.M. Helicobacter pylori: epidemiology and routes of transmission. Epidemiol Rev. 2000, 22(2):283-97. [DOI via Crossref]   

This Article Cited By the following articles

Gastrocolocutaneous Fistula- Delayed complication of Percutaneous Endoscopic Gastrostomy, Case report
THE ULUTAS MEDICAL JOURNAL 2015; (): 1.

1
 

How to Cite this Article
Pubmed Style

Ahamed F, Chandrappa N, Abdallah EM. A comparative study of diagnosis methods for detection of Helicobacter pylori in gastro-duodenal diseases in Human. Ulutas Med J. 2015; 1(3): 64-67. doi:10.5455/umj.20151009012034



Web Style

Ahamed F, Chandrappa N, Abdallah EM. A comparative study of diagnosis methods for detection of Helicobacter pylori in gastro-duodenal diseases in Human. www.scopemed.org/?mno=203103 [Access: November 24, 2017]. doi:10.5455/umj.20151009012034



AMA (American Medical Association) Style

Ahamed F, Chandrappa N, Abdallah EM. A comparative study of diagnosis methods for detection of Helicobacter pylori in gastro-duodenal diseases in Human. Ulutas Med J. 2015; 1(3): 64-67. doi:10.5455/umj.20151009012034



Vancouver/ICMJE Style

Ahamed F, Chandrappa N, Abdallah EM. A comparative study of diagnosis methods for detection of Helicobacter pylori in gastro-duodenal diseases in Human. Ulutas Med J. (2015), [cited November 24, 2017]; 1(3): 64-67. doi:10.5455/umj.20151009012034



Harvard Style

Ahamed, F., Chandrappa, N. & Abdallah, E. M. (2015) A comparative study of diagnosis methods for detection of Helicobacter pylori in gastro-duodenal diseases in Human. Ulutas Med J, 1 (3), 64-67. doi:10.5455/umj.20151009012034



Turabian Style

Ahamed, Fiaz, N.R. Chandrappa, and Emad M. Abdallah. 2015. A comparative study of diagnosis methods for detection of Helicobacter pylori in gastro-duodenal diseases in Human. THE ULUTAS MEDICAL JOURNAL, 1 (3), 64-67. doi:10.5455/umj.20151009012034



Chicago Style

Ahamed, Fiaz, N.R. Chandrappa, and Emad M. Abdallah. "A comparative study of diagnosis methods for detection of Helicobacter pylori in gastro-duodenal diseases in Human." THE ULUTAS MEDICAL JOURNAL 1 (2015), 64-67. doi:10.5455/umj.20151009012034



MLA (The Modern Language Association) Style

Ahamed, Fiaz, N.R. Chandrappa, and Emad M. Abdallah. "A comparative study of diagnosis methods for detection of Helicobacter pylori in gastro-duodenal diseases in Human." THE ULUTAS MEDICAL JOURNAL 1.3 (2015), 64-67. Print. doi:10.5455/umj.20151009012034



APA (American Psychological Association) Style

Ahamed, F., Chandrappa, N. & Abdallah, E. M. (2015) A comparative study of diagnosis methods for detection of Helicobacter pylori in gastro-duodenal diseases in Human. THE ULUTAS MEDICAL JOURNAL, 1 (3), 64-67. doi:10.5455/umj.20151009012034








AUTHOR LOGIN

REVIEWER LOGIN

Indexed
&
Abstracted


CrossRef

ScopeMed

Google Scholar

TUBITAK Dergipark

Index Scholar

Index Copernicus


ABOUT JOURNAL
POLICIES and STATEMENTS

The articles in Ulutas Medical Journal are open access articles licensed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-sa/3.0/) which permits unrestricted, non-commercial use, distribution and reproduction in any medium, provided the work is properly cited.