E-ISSN 2149-388X | ISSN 2149-0430
 

Original Article 


Prevalence of Brucella Melitensis and Brucella Abortus in Raw Milk and Dairy Product by Real Time PCR Technique

Yaran Majid, Najafi Somayeh, Shoaei Parisa, Ataei Behrooz, Fadaei Nobari Reza, Ramazanpour Javad, Farsi Mehdi, Ahmadi Ahwaz Nasrin, Khorasani Marzieh.

Cited By:8

Abstract
Introduction: Brucellosis is a public health problem with a high incidence. The main causes of acquiring brucellosis are through the consumption of unpasteurized milk and dairy products. In this study we described a real-time PCR assay for more sensitive, rapid detection and differentiation of Brucella.abortus and Brucella.melitensis in raw milk and dairy products in Isfahan province.
Materials and Methods: A Taq Man analysis and single step PCR (BruAb2_0168BMEII0466 genes) has performed in total of 132 unpasteurized milk samples and 65 dairy products. Samples were selected from various markets and animal farms located in different cities of Isfahan province. The duration period of sample collection was nearly two months (May to June 2012).
Results: By real-time PCR 4 of 132 milk specimens (3%) were positive for B. melitensis. Also one oil sample between 65 different dairy products (1.5%) was positive for B. melitensis. None of the specimens were positive for B. abortus. We detected direct and incomplete correlation between brucella infected milk and different cities(r=0.024, p value >0.05).
Conclusion: Results of present study indicated a relative high presence of this pathogen in raw milk. Real-time PCR is technically more simple, accurate, and rapid than current standard methods for identification of Brucella species .One of the most effective routes to control the disease includes pasteurization or boiling of milk and other dairy products for human consumption.

Key words: Brucellosis, real time PCR, milk


 
ARTICLE TOOLS
Abstract
PDF Fulltext
How to cite this articleHow to cite this article
Citation Tools
Related Records
 Articles by Yaran Majid
Articles by Najafi Somayeh
Articles by Shoaei Parisa
Articles by Ataei Behrooz
Articles by Fadaei Nobari Reza
Articles by Ramazanpour Javad
Articles by Farsi Mehdi
Articles by Ahmadi Ahwaz Nasrin
Articles by Khorasani Marzieh
on Google
on Google Scholar


How to Cite this Article
Pubmed Style

Yaran Majid, Najafi Somayeh, Shoaei Parisa, Ataei Behrooz, Fadaei Nobari Reza, Ramazanpour Javad, Farsi Mehdi, Ahmadi Ahwaz Nasrin, Khorasani Marzieh. Prevalence of Brucella Melitensis and Brucella Abortus in Raw Milk and Dairy Product by Real Time PCR Technique. Ulutas Med J. 2016; 2(1): 7-11. doi:10.5455/umj.20151004054235


Web Style

Yaran Majid, Najafi Somayeh, Shoaei Parisa, Ataei Behrooz, Fadaei Nobari Reza, Ramazanpour Javad, Farsi Mehdi, Ahmadi Ahwaz Nasrin, Khorasani Marzieh. Prevalence of Brucella Melitensis and Brucella Abortus in Raw Milk and Dairy Product by Real Time PCR Technique. https://www.ulutasmedicaljournal.com/?mno=201961 [Access: December 05, 2023]. doi:10.5455/umj.20151004054235


AMA (American Medical Association) Style

Yaran Majid, Najafi Somayeh, Shoaei Parisa, Ataei Behrooz, Fadaei Nobari Reza, Ramazanpour Javad, Farsi Mehdi, Ahmadi Ahwaz Nasrin, Khorasani Marzieh. Prevalence of Brucella Melitensis and Brucella Abortus in Raw Milk and Dairy Product by Real Time PCR Technique. Ulutas Med J. 2016; 2(1): 7-11. doi:10.5455/umj.20151004054235



Vancouver/ICMJE Style

Yaran Majid, Najafi Somayeh, Shoaei Parisa, Ataei Behrooz, Fadaei Nobari Reza, Ramazanpour Javad, Farsi Mehdi, Ahmadi Ahwaz Nasrin, Khorasani Marzieh. Prevalence of Brucella Melitensis and Brucella Abortus in Raw Milk and Dairy Product by Real Time PCR Technique. Ulutas Med J. (2016), [cited December 05, 2023]; 2(1): 7-11. doi:10.5455/umj.20151004054235



Harvard Style

Yaran Majid, Najafi Somayeh, Shoaei Parisa, Ataei Behrooz, Fadaei Nobari Reza, Ramazanpour Javad, Farsi Mehdi, Ahmadi Ahwaz Nasrin, Khorasani Marzieh (2016) Prevalence of Brucella Melitensis and Brucella Abortus in Raw Milk and Dairy Product by Real Time PCR Technique. Ulutas Med J, 2 (1), 7-11. doi:10.5455/umj.20151004054235



Turabian Style

Yaran Majid, Najafi Somayeh, Shoaei Parisa, Ataei Behrooz, Fadaei Nobari Reza, Ramazanpour Javad, Farsi Mehdi, Ahmadi Ahwaz Nasrin, Khorasani Marzieh. 2016. Prevalence of Brucella Melitensis and Brucella Abortus in Raw Milk and Dairy Product by Real Time PCR Technique. THE ULUTAS MEDICAL JOURNAL, 2 (1), 7-11. doi:10.5455/umj.20151004054235



Chicago Style

Yaran Majid, Najafi Somayeh, Shoaei Parisa, Ataei Behrooz, Fadaei Nobari Reza, Ramazanpour Javad, Farsi Mehdi, Ahmadi Ahwaz Nasrin, Khorasani Marzieh. "Prevalence of Brucella Melitensis and Brucella Abortus in Raw Milk and Dairy Product by Real Time PCR Technique." THE ULUTAS MEDICAL JOURNAL 2 (2016), 7-11. doi:10.5455/umj.20151004054235



MLA (The Modern Language Association) Style

Yaran Majid, Najafi Somayeh, Shoaei Parisa, Ataei Behrooz, Fadaei Nobari Reza, Ramazanpour Javad, Farsi Mehdi, Ahmadi Ahwaz Nasrin, Khorasani Marzieh. "Prevalence of Brucella Melitensis and Brucella Abortus in Raw Milk and Dairy Product by Real Time PCR Technique." THE ULUTAS MEDICAL JOURNAL 2.1 (2016), 7-11. Print. doi:10.5455/umj.20151004054235



APA (American Psychological Association) Style

Yaran Majid, Najafi Somayeh, Shoaei Parisa, Ataei Behrooz, Fadaei Nobari Reza, Ramazanpour Javad, Farsi Mehdi, Ahmadi Ahwaz Nasrin, Khorasani Marzieh (2016) Prevalence of Brucella Melitensis and Brucella Abortus in Raw Milk and Dairy Product by Real Time PCR Technique. THE ULUTAS MEDICAL JOURNAL, 2 (1), 7-11. doi:10.5455/umj.20151004054235